Is your article EV-TRACKed?

The EV-TRACK knowledgebase is developed to cope with the need for transparency and rigour to increase reproducibility and facilitate standardization of extracellular vesicle (EV) research. The knowledgebase includes a checklist for authors and editors intended to improve the transparency of methodological aspects of EV experiments, allows queries and meta-analysis of EV experiments and keeps track of the current state of the art. Widespread implementation by the EV research community is key to its success

Counting matroids in minor-closed classes

A flat cover is a collection of flats identifying the non-bases of a matroid. We introduce the notion of cover complexity, the minimal size of such a flat cover, as a measure for the complexity of a matroid, and present bounds on the number of matroids on $n$ elements whose cover complexity is bounded. We apply cover complexity to show that the class of matroids without an $N$-minor is asymptotically small in case $N$ is one of the sparse paving matroids $U_{2,k}$, $U_{3,6}$, $P_6$, $Q_6$, or $R_6$, thus confirming a few special cases of a conjecture due to Mayhew, Newman, Welsh, and Whittle. On the other hand, we show a lower bound on the number of matroids without $M(K_4)$-minor which asymptoticaly matches the best known lower bound on the number of all matroids, due to Knuth.Comment: 13 pages, 3 figure

On the number of matroids

We consider the problem of determining $m_n$, the number of matroids on $n$ elements. The best known lower bound on $m_n$ is due to Knuth (1974) who showed that $\log \log m_n$ is at least $n-3/2\log n-1$. On the other hand, Piff (1973) showed that $\log\log m_n\leq n-\log n+\log\log n +O(1)$, and it has been conjectured since that the right answer is perhaps closer to Knuth's bound. We show that this is indeed the case, and prove an upper bound on $\log\log m_n$ that is within an additive $1+o(1)$ term of Knuth's lower bound. Our proof is based on using some structural properties of non-bases in a matroid together with some properties of independent sets in the Johnson graph to give a compressed representation of matroids.Comment: Final version, 17 page

An entropy argument for counting matroids

We show how a direct application of Shearers' Lemma gives an almost optimum bound on the number of matroids on $n$ elements.Comment: Short note, 4 page

Scaling-up vaccine production: implementation aspects of a biomass growth observer and controller

Abstract This study considers two aspects of the implementation of a biomass growth observer and specific growth rate controller in scale-up from small- to pilot-scale bioreactors towards a feasible bulk production process for whole-cell vaccine against whooping cough. The first is the calculation of the oxygen uptake rate, the starting point for online monitoring and control of biomass growth, taking into account the dynamics in the gas-phase. Mixing effects and delays are caused by amongst others the headspace and tubing to the analyzer. These gas phase dynamics are modelled using knowledge of the system in order to reconstruct oxygen consumption. The second aspect is to evaluate performance of the monitoring and control system with the required modifications of the oxygen consumption calculation on pilot-scale. In pilot-scale fed-batch cultivation good monitoring and control performance is obtained enabling a doubled concentration of bulk vaccine compared to standard batch productio

e-Learning cookbook. TPACK in professional development in higher education

Information and communication technology (ICT) makes it possible to bring information to everyone who wants to learn. Rapid advances in technology offer strong support for using ICT in teaching. Online education can intensify and improve students' learning process, and enables us to reach more students than by traditional means. The number of courses and modules being offered online is increasing rapidly worldwide. Although online education can reach more people nowadays and new and challenging learning experiences can be created with it, in the average university course the digital dimension too often remains limited to simply publishing the existing face-to-face course content online. It is crucial that lecturers have and can obtain knowledge about how to design technology-enhanced teaching. Technical advances can be expected to continue in the future, and those who wish to implement educational technology in their own teaching practice must reckon on becoming lifelong learners. This fits the culture of academic teachers perfectly: they are already lifelong learners and creators of new knowledge within their discipline. This book is based on the notion that a lecturer who uses ICT in teaching must learn how to apply his or her knowledge about content, pedagogy and technology in an integrated manner. The idea of integrating these three types of knowledge is based on the TPACK model, which stands for Technological Pedagogical Content Knowledge model. The material for this book was developed in a Dutch higher education innovation project known as MARCHET (Make Relevant Choices in Educational Technology, MARCHET, 2009-2011)

e-Learning cookbook. TPACK in professional development in higher education

Information and communication technology (ICT) makes it possible to bring information to everyone who wants to learn. Rapid advances in technology offer strong support for using ICT in teaching. Online education can intensify and improve students' learning process, and enables us to reach more students than by traditional means. The number of courses and modules being offered online is increasing rapidly worldwide. Although online education can reach more people nowadays and new and challenging learning experiences can be created with it, in the average university course the digital dimension too often remains limited to simply publishing the existing face-to-face course content online. It is crucial that lecturers have and can obtain knowledge about how to design technology-enhanced teaching. Technical advances can be expected to continue in the future, and those who wish to implement educational technology in their own teaching practice must reckon on becoming lifelong learners. This fits the culture of academic teachers perfectly: they are already lifelong learners and creators of new knowledge within their discipline. This book is based on the notion that a lecturer who uses ICT in teaching must learn how to apply his or her knowledge about content, pedagogy and technology in an integrated manner. The idea of integrating these three types of knowledge is based on the TPACK model, which stands for Technological Pedagogical Content Knowledge model. The material for this book was developed in a Dutch higher education innovation project known as MARCHET (Make Relevant Choices in Educational Technology, MARCHET, 2009-2011)

Economic evaluation of orphan drug Lutetium-Octreotate vs. Octreotide long-acting release for patients with an advanced midgut neuroendocrine tumour in the Netherlands

OBJECTIVES: Multiple studies showed positive effects of Lutetium-Octreotate (LO) treatment in neuroendocrine tumours. LO has been used in the Netherlands since the 1980s and recently received the orphan status shortly after the acquisition by Novartis. Since then, the official list price has increased sixfold. From a value-based pricing perspective, we analysed the impact of the increase in price on the incremental cost-effectiveness ratio (ICER) of LO treatment compared to optimal best supportive care, a high dose of Octreotide long-acting release (O-LAR), using the clinical data of the NETTER-1 trial. METHODS: A Markov model was developed to evaluate the costs per quality-adjusted life-year (QALY) for LO treatment compared to O-LAR from the healthcare perspective. A scenario analysis was conducted to compare the cost-effectiveness with the initial and increased price level of the LO-treatment. RESULTS: At the increased price level, the cost-effectiveness analysis rendered a deterministic ICER of €53,500 per QALY, while at the initial pricing, the ICER was €19,000 per QALY. The probabilistic sensitivity analysis (PSA) showed that LO had a high probability of being cost-effective at both the increased and initial price level, considering a cost-effectiveness threshold of €80,000. CONCLUSIONS: Even at the increased price level, LO treatment can still be considered cost-effective using the applicable Dutch willingness-to-pay threshold of 80,000 euro per QALY. Considering the public scrutiny in relation to this price increase, these outcomes raise the question whether traditional cost-effectiveness methods are sufficient in fully capturing the societal acceptance of prices of new medicines. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s10198-021-01303-2

Health economic evaluation of current vaccination strategies and new vaccines against tuberculosis:a systematic review

Introduction: Bacillus Calmette-Guerin (BCG) is the only licensed vaccine for tuberculosis, but its effectiveness is limited and varies by age. New candidate vaccines are currently being investigated. In response to the declining incidence of TB, practices relating to BCG vaccination have changed in various countries in recent years. A valid cost-effectiveness study is therefore needed in order to assist decision-makers in the implementation of cost-effective strategies for BCG vaccination. Areas covered: Studies involving economic evaluations of BCG vaccination were reviewed in order to present current findings concerning a range of BCG vaccination strategies in a variety of regions, target populations, and vaccine types. The Quality of Health Economic Studies (QHES) instrument was used to assess the quality of the studies included in the analysis. Expert opinion: Most of the studies showed a favorable economic profile of BCG vaccination. Selective strategies seem the most cost-effective option for low-incidence areas. Varying results on revaccination strategies did not lead to any conclusive finding on the cost-effectiveness of the strategies. A novel vaccine - either a BCG replacement or booster vaccine that provides better protection, especially in adults - has the potential to enhance the cost-effectiveness of vaccinating against tuberculosis

Utilization of the Cepheid Xpert® CT/NG Sample Adequacy Control to Determine the Influence of the Urethral Swab on Cellular Content in Post-Swab versus Pre-Swab Urine

Chlamydia trachomatis/Neisseria gonorrhoeae assay performance in males is typically determined using post-swab urine, though pre-swab urine is used in practice. We collected swabs and urine from men and used the Cepheid Xpert® CT/NG sample adequacy control to determine the effect of swab collection on urine cellular content. No difference was observed